Thermo Fisher Scientific MCP-4 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL
• Publications: 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-4 matrix.

Sandwich ELISA:
To detect Human MCP-4 (CCL13) by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-4 (CCL13) (500-P04BT) as a detection antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human MCP-4 (CCL13).

Western Blot:
To detect Human MCP-4 (CCL13) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human MCP-4 (CCL13) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFα. MCP-4 is a ligand for three different G protein coupled receptors: CCR2, CCR3, and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils, and basophils, and this signaling is associated with the allergic response.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일: 500-P04-100UG_MCP-4_Q99616-1_Rabbit.svg, 500-P04-100UG_MCP-4_Q99616-1_Rabbit_PDP.jpeg)