Thermo Fisher Scientific MCP-4 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL
• Publications: 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human MCP-4 (CCL13).
Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-4 matrix.

Sandwich ELISA:
To detect Human MCP-4 (CCL13) by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required.
This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-4 (CCL13) (500-P04GBT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human MCP-4 (CCL13).

Western Blot:
To detect Human MCP-4 (CCL13) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Human MCP-4 (CCL13) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFα.
MCP-4 is a ligand for three different G protein coupled receptors: CCR2, CCR3, and CCR5.
It activates signaling in monocytes, T lymphocytes, eosinophils, and basophils, and this signaling is associated with allergic response.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.