Thermo Fisher Scientific c-Myc Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid repeated freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. The product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This antiserum is directed against human c-Myc and is useful for detecting its presence in various assays. Anti-c-Myc is highly specific and shows no cross-reaction with N-Myc or L-Myc proteins.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays an essential role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK regulates its activity in cell proliferation and cycle control. c-Myc is critical for vasculogenesis and angiogenesis in tumor development.

The c-Myc oncogene (p62 c-Myc) regulates normal cellular proliferation and differentiation, while its deregulated expression can induce apoptosis. Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to either amino- or carboxy-termini of target proteins.

Amplification of the c-Myc gene has been observed in several human tumors, including lung, breast, and colon carcinomas. N-Myc amplification is associated with neuroblastomas. Translocation of the c-Myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell lymphoproliferative disorders.

Aberrant c-Myc expression has been reported in tumors of various origins, including colorectal, gastric, hepatic, mammary, ovarian, pulmonary, prostatic, thyroidal, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.