Thermo Fisher Scientific Gli1 Polyclonal Antibody
Thermo Fisher Scientific의 Gli1 Polyclonal Antibody는 인간 및 마우스 Gli-1 단백질을 인식하는 토끼 유래 폴리클로날 항체로, WB, IHC, ELISA에 최적화되어 있습니다. 고농도(75 mg/mL) 액상 형태이며, -20°C에서 보관 가능합니다. 연구용으로만 사용됩니다.
- 카탈로그번호
- 100-401-223x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Gli1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805–820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 75 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- 보관 전 -20°C에서 보관하십시오.
- 장기 보관 시 소분 후 -20°C 이하에서 냉동 보관하십시오.
- 반복적인 동결 및 해동은 피하십시오.
- 상온에서 방치 후 투명하지 않은 경우 원심분리하여 사용하십시오.
- 4°C에서 수 주간 안정적입니다(희석 전 액상 상태).
- 즉시 사용 전 희석하십시오.
Reactivity is observed against Mouse and Human Gli-1. Cross-reactivity with other species is likely but has not been determined. No reaction occurs with human or mouse Gli-2 or Gli-3.
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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