Thermo Fisher Scientific CD8 alpha Recombinant Rabbit Monoclonal Antibody (23GB1420)
CD8α 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, HEK293F 발현 시스템을 사용했습니다. 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB1420 |
| Immunogen | A synthesized peptide derived from human CD8 (200–235 AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein belonging to the immunoglobulin family of receptors. It plays a critical role in signal transduction, T cell differentiation, and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules. The CD8α chain is essential for MHC-I binding and is also expressed as a homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement of MHC-I/peptide complexes by CD8 triggers recruitment of Lck kinase, leading to lymphokine production, cell motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for pathogen and tumor cell clearance, and their differentiation is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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