Thermo Fisher Scientific MEKK3 Recombinant Rabbit Monoclonal Antibody (23GB2870)
MEKK3 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. HEK293F 발현 시스템 기반, 고순도 친화 크로마토그래피 정제. 인간, 생쥐, 랫트 반응성. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB2870 |
| Immunogen | A synthesized peptide derived from human MEKK3 (1–47AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and ERK pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: MA5-52524_MEKK3_Q99759-1_Rabbit.svg, MA5-52524_MEKK3_Q99759-1_Rabbit_PDP.jpeg)
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