Thermo Fisher Scientific NOX2 Recombinant Rabbit Monoclonal Antibody (SN07-16)
Recombinant rabbit monoclonal antibody targeting NOX2 (gp91-phox) for WB applications. High specificity and sensitivity with consistent lot-to-lot performance. Suitable for human, mouse, and rat samples. Formulated for stable storage and reliable resea...
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Applications
- Western Blot (WB): Tested dilution 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN07-16 |
| Immunogen | Synthetic peptide within Human NOX2 (aa 131–180) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2809581 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Mox1 and gp91-phox (NOX2) are key components of the NADPH oxidase complex, responsible for superoxide generation in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells.
The complex includes:
- Membrane-bound flavocytochrome (gp91-phox and p22-phox)
- Cytosolic proteins (p47-phox, p67-phox)
Both gp91-phox and p22-phox act as O₂ sensors initiating signaling under hypoxic conditions.
gp91-phox is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes, while Mox1 is mainly detected in the colon, with low expression in uterus and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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