Thermo Fisher Scientific Papain Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,500

-

Immunohistochemistry (IHC)

1:200- 1:1,000

-

ELISA (ELISA)

1:1,000-1:5,000

-

Immunoprecipitation (IP)

1:500- 1:2,500

-

Product Specifications

Species Reactivity

Plant

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% gentamicin sulfate

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]. Cross reactivity against Papain from other sources may occur but has not been specifically determined.

Target Information

Papain is a protease derived from the papaya fruit (Carica papaya). It hydrolyses peptides with broad specificity, but shows a preference for large hydrophobic amino acids in the P2 position. It is synthesised as an inactive 40 kDa precursor containing a 107 residue N terminal pro region and a 26 residue signal peptide.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.