Thermo Fisher Scientific Papain Polyclonal Antibody, HRP
Thermo Fisher Scientific의 Papain Polyclonal Antibody, HRP는 식물 유래 파파인 단백질에 특이적인 염소 다클론 항체입니다. HRP 결합형으로 Western blot, IHC, ELISA, IP에 사용 가능하며, 고순도 이온교환 크로마토그래피로 정제되었습니다. 연구용으로 안정적이며 장기 보관 시 -20°C에서 유지됩니다.
- 카탈로그번호
- 200-1374xxxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Papain Polyclonal Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution Range |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,500 |
| Immunohistochemistry (IHC) | 1:200 – 1:1,000 |
| ELISA | 1:1,000 – 1:5,000 |
| Immunoprecipitation (IP) | 1:500 – 1:2,500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Plant |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya. |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Papain is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, as well as purified and partially purified Papain (Carica papaya). Cross-reactivity against Papain from other sources may occur but has not been specifically determined.
Target Information
Papain is a protease derived from the papaya fruit (Carica papaya). It hydrolyzes peptides with broad specificity, showing preference for large hydrophobic amino acids in the P2 position. It is synthesized as an inactive 40 kDa precursor containing a 107 residue N-terminal pro region and a 26 residue signal peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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