Thermo Fisher Scientific Lactate Dehydrogenase Polyclonal Antibody, HRP

Applications 및 Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Product is stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion exchange chromatography followed by extensive dialysis against the stated buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified Lactate Dehydrogenase [Rabbit Muscle].
Cross-reactivity against LDH from other tissues and species may occur but has not been specifically determined.

Target Information

Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
Five LDH isoenzymes exist based on the proportion of M and H chains in the tetrameric structure.
LDHA is hypoxia-inducible and regulated by HIF1α activity, while LDHB is upregulated in many cancers.
Mutations in LDHA can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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