
Thermo Fisher Scientific Carbonic Anhydrase I Polyclonal Antibody, HRP
Carbonic Anhydrase I에 특이적인 HRP 결합 폴리클로날 항체로 인간 적혈구 유래 항원을 인식합니다. Western blot, IHC, ELISA, IP 등에 사용 가능하며, 정제된 IgG 형태로 제공됩니다. 연구용으로만 사용됩니다.
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- 200-1354xxxxx (2개 옵션)
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Thermo Fisher Scientific Carbonic Anhydrase I Polyclonal Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,500 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carbonic Anhydrase I (Human Erythrocytes) |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Carbonic Anhydrase I antibody is purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified Carbonic Anhydrase I (Human Erythrocytes).
Cross-reactivity with Carbonic Anhydrase I from other tissues or species may occur but has not been specifically determined.
Target Information
Carbonic anhydrases (CAs) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.
They are involved in respiration, calcification, acid-base balance, bone resorption, and formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
CA1 encodes a cytosolic protein highly expressed in erythrocytes and is closely linked to CA2 and CA3 genes on chromosome 8.
Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 200-1354-0100_Carbonic_Anhydrase_I_P00915-1_Goat.svg, 200-1354-0100_Carbonic_Anhydrase_I_P00915-1_Goat_PDP.jpeg)
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