Thermo Fisher Scientific 5-Methylcytosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

25-100 µg/mL

-

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Published species

Not Applicable

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

5-methyl cytosine molecule conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3 mg/mL

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1957442

Product Specific Information

The use of 7mM NaOH in the IP is crutial.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.