Thermo Fisher Scientific ARC Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Recommended positive control: HeLa whole cell lysate
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms.

• Isoform 1: Involved in RNA splicing.
• Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death.

ARC inhibits extrinsic apoptotic pathways in two ways:

1. Interacts with FAS and FADD upon FAS activation, blocking death-inducing signaling complex (DISC) assembly.
2. Interacts with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting soluble CASP8 available for DISC-mediated activation.

ARC also inhibits intrinsic apoptotic pathways in response to various stresses through interactions with BAX, leading to BAX inactivation and prevention of mitochondrial dysfunction and release of pro-apoptotic factors.
Mutations affecting this gene can result in myoclonus, familial cortical.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.