Thermo Fisher Scientific PDE4A Monoclonal Antibody (OTI3D4), TrueMAB
PDE4A 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow cytometry에 사용 가능. HEK293T 세포에서 발현된 full-length human PDE4A 단백질을 면역원으로 제작. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D4 |
| Immunogen | Full length human recombinant protein of human PDE4A produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cyclic nucleotides act as second messengers mediating cellular responses to hormones, light, and neurotransmitters.
Cyclic nucleotide phosphodiesterases (PDEs) regulate intracellular cyclic nucleotide levels and play key roles in signal transduction.
PDE4A is a class IV cAMP-specific phosphodiesterase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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