Thermo Fisher Scientific RACK1 Polyclonal Antibody
RACK1 단백질을 인식하는 Rabbit Polyclonal 항체. IHC(P) 검증 완료, 1:100 희석 권장. PKC 신호전달 관련 연구에 적합. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from internal region of human RACK-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2550034 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is prostate carcinoma.
Target Information
RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms.
Its main function is to recruit PKC and various other proteins to specific locations to form multiprotein complexes, mediating various signal pathways.
RACK1 is required for VANGL2 membrane localization and for PTK2/FAK1 phosphorylation and dephosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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