Thermo Fisher Scientific S6 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma tissue.

Target Information

Ribosomes are organelles that catalyze protein synthesis and consist of a small 40S subunit and a large 60S subunit. Together, these subunits are composed of four RNA species and approximately 80 distinct proteins.
The S6 ribosomal protein is a component of the 40S subunit and belongs to the S6E family. It is a major substrate of ribosomal protein kinases, with phosphorylation occurring on five C-terminal serine residues in response to stimuli such as growth factors, tumor-promoting agents, and mitogens.
Dephosphorylation occurs during growth arrest, suggesting a role in regulating cell growth and proliferation through selective translation of specific mRNA classes.
Multiple processed pseudogenes of this gene are dispersed throughout the genome.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.