Thermo Fisher Scientific PSMA Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100
• Publications: [References not provided]

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is prostate carcinoma.

Target Information

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family.
The protein acts as a glutamate carboxypeptidase on various substrates, including the nutrient folate and the neuropeptide N-acetyl-L-aspartyl-L-glutamate.
It is expressed in multiple tissues such as prostate, central and peripheral nervous system, and kidney.
Mutations may cause impaired intestinal absorption of dietary folates, leading to low blood folate levels and hyperhomocysteinemia.
Expression in the brain may be involved in pathological conditions associated with glutamate excitotoxicity.
In the prostate, the protein is up-regulated in cancerous cells and serves as an effective diagnostic and prognostic indicator of prostate cancer.
This gene likely originated from a duplication event of a nearby chromosomal region.
Alternative splicing produces multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.