Thermo Fisher Scientific CD34 Recombinant Mouse Monoclonal Antibody (2E 10)
CD34 단백질을 인식하는 마우스 재조합 단일클론 항체로, Western blot, ICC/IF, Flow Cytometry에 사용 가능. 인간 CD34에 특이적으로 반응하며, HEK293 세포에서 발현됨. Protein A로 정제된 액상 형태로 제공되며, 4°C 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG3, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2E 10 |
| Immunogen | The antibody was raised by repeatedly immunising BALB/c mice with KG-1a cells. Target: CD34 (UniProt ID: P28906-1), Antigen range: 1–385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
Product Specific Information
The antibody is specific to a class III epitope on CD34, which is not susceptible to cleavage by neuraminidase, chymopapain, or glycoprotease.
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on many stem cell populations. It functions as a stem cell marker, with expression reversible on human hematopoietic stem cells.
CD34 may act as a surface receptor involved in receptor-mediated endocytosis and regulation of adhesion, differentiation, and proliferation of hematopoietic stem and progenitor cells.
It is believed to facilitate attachment of stem cells to the bone marrow extracellular matrix or stromal cells and may present carbohydrate ligands to selectins. The intracellular domain can be phosphorylated by activated protein kinase C, suggesting a role in signal transduction.
Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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