Thermo Fisher Scientific Leptin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:50 - 1:200

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ELISA (ELISA)

-

View 1 publication 1 publication

ChIP assay (ChIP)

1.5 µg/1x10^6 cells

-

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Sheep, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Leptin,uniProtId:P41160-1,ncbiNodeId:10090,antigenRange:26-44,antigenLength:167,antigenImageFileName:PA1-051_Leptin_P41160-1_Rabbit.svg,antigenImageFileNamePDP:PA1-051_Leptin_P41160-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325786

Product Specific Information

PA1-051 detects leptin from human, mouse, sheep and rat samples.

PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus.

The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine.

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin`s effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.