Thermo Fisher Scientific PKM2 Polyclonal Antibody
Rabbit polyclonal antibody targeting PKM2, suitable for WB, IHC, and IP applications. Recognizes PKM2 across multiple species. Recommended shelf life 1 year, stored at 4°C. Unconjugated, liquid form, concentration 1 mg/mL.
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Thermo Fisher Scientific PKM2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Between amino acids 481–531 of PKM2 (UniProt ID: P14618-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, X. laevis, Chicken, Rabbit, Cat, and Orangutan
Target Information
PKM2 (Pyruvate Kinase M2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP. It interacts with thyroid hormone, mediating metabolic effects, and also binds Opa protein, suggesting a role in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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