Thermo Fisher Scientific PKM2 Polyclonal Antibody
PKM2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. Human PKM2의 1–50번 잔기 영역을 면역원으로 제작. 액상 형태, 1 mg/mL 농도, 4°C 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Item | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of Human Pyruvate Kinase Isozymes M1/M2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
PKM2 (Pyruvate Kinase M2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP.
It interacts with thyroid hormone, mediating metabolic effects induced by thyroid hormones.
PKM2 also binds to the Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells, suggesting a potential role in bacterial pathogenesis.
Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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