Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), PE, eBioscience

Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• PE-Cyanine7
• Super Bright 436
• Custom conjugation available

Product Specific Information

The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. The Toll family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain homologous to the type I IL-1 receptor. TLR2 and TLR4 function as pattern recognition receptors involved in innate immunity and inflammation.

TLR4 associates with MD-2 and, together with CD14, forms the complex responsible for LPS recognition and signaling. In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages, and LPS incubation results in down-regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, known for LPS hyporesponsiveness.

MTS510 co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), preferentially reacting with the TLR4/MD-2 complex. In contrast, UT41 binds regardless of complex formation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells at ≤0.5 µg/test (100 µL final volume). Optimal antibody titration is recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MD-2 belongs to the Toll/interleukin-1 receptor (TIR) family, including TLRs that recognize pathogen-associated molecular patterns (PAMPs) and link innate and adaptive immunity. TLR4, the main receptor for lipopolysaccharide (LPS), requires MD-2 binding for maximal response. The specificity of LPS recognition varies by MD-2 species; e.g., human MD-2 in mouse cells can alter TLR4 reactivity to LPS analogs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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