Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), PE, eBioscience
TLR4/MD-2 복합체를 인식하는 Mouse용 단클론 항체로, PE 형광 표지되어 Flow Cytometry에 적합합니다. 0.5 µg/test로 최적화되어 있으며, 면역반응 및 염증 연구에 활용됩니다. PBS 기반 용액으로 4°C 암소 보관하며 동결 금지.
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Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 27 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MTS510 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466263 |
Available Formats
- Unconjugated
- APC
- Biotin
- Functional Grade
- PE-Cyanine7
- Super Bright 436
- Custom conjugation available
Product Specific Information
The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. The Toll family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain homologous to the type I IL-1 receptor. TLR2 and TLR4 function as pattern recognition receptors involved in innate immunity and inflammation.
TLR4 associates with MD-2 and, together with CD14, forms the complex responsible for LPS recognition and signaling. In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages, and LPS incubation results in down-regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, known for LPS hyporesponsiveness.
MTS510 co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), preferentially reacting with the TLR4/MD-2 complex. In contrast, UT41 binds regardless of complex formation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells at ≤0.5 µg/test (100 µL final volume). Optimal antibody titration is recommended.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
MD-2 belongs to the Toll/interleukin-1 receptor (TIR) family, including TLRs that recognize pathogen-associated molecular patterns (PAMPs) and link innate and adaptive immunity. TLR4, the main receptor for lipopolysaccharide (LPS), requires MD-2 binding for maximal response. The specificity of LPS recognition varies by MD-2 species; e.g., human MD-2 in mouse cells can alter TLR4 reactivity to LPS analogs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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