Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells.
CD28 serves as a ligand for CD80 (B7-1) and CD86 (B7-2), providing a potent co-stimulatory signal for T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and increases cytotoxicity of CD3-activated T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells. Recommended use at 5 µL (1.0 µg) per test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.

Fluorochrome: Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for instruments with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C (dark) using:

• IC Fixation Buffer (Product # 00-8222-49), or
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Methanol Compatibility: Short-term methanol fixation is compatible, but avoid long-term storage in methanol-containing buffers.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T-cell surface glycoprotein of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and provides a crucial co-stimulatory signal by binding to CD80 and CD86 on antigen-presenting cells.
CD28 prevents T cells from entering an anergic or apoptotic state and is vital for proper immune activation. Expression patterns vary across thymocyte maturation stages.
Dysfunction of CD28 is associated with diseases such as mycosis fungoides and Sézary’s disease.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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