Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• Alexa Fluor Plus 555
• Alexa Fluor Plus 750
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 661
• Brilliant Violet 421 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 660-120S / 800
• NovaFluor Red 710
• NovaFluor UV 765
• NovaFluor Violet 690 / 800
• NovaFluor Yellow 610 / 660 / 810
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation

Product Specific Information

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ and CD8+ T lymphocytes and B cells. CD45RA+ T cell populations are mainly naive/virgin, allowing use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested by flow cytometry using normal human peripheral blood cells
• Recommended Usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401)
• or Brilliant Stain Buffer (Product # 00-4409)
  to minimize non-specific interactions.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples may be stored in:

• IC Fixation Buffer (Product # 00-8222)
• or 1-step Fix/Lyse Solution (Product # 00-5333)
  for up to 3 days at 4°C in the dark.

Methanol Compatibility:
BUV737 is compatible with short-term methanol-based fixation but should not be stored in methanol-containing buffers longer than 1 hour.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45RA is an isoform of the CD45 complex, a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells.
It plays a key role in lymphocyte development, antigen signaling, and integrin-mediated signal transduction, and is involved in apoptosis regulation.
CD45 isoforms differ due to alternative splicing and vary across lymphoid subtypes.
Dysfunction of CD45 is associated with severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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