Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 510 / 610-30S / 660-40S
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702
• Custom conjugation available

Product Specific Information

The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.1-expressing mouse strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes, ≤ 0.5 µg per test (100 µL final volume)

Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye emitting at 732 nm, designed for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility before use.

Staining and Buffer Recommendations

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401)
• Brilliant Stain Buffer (Product # 00-4409)

These buffers help minimize non-specific polymer interactions.

Handling and Fixation

• Light Sensitivity: Protect vial and samples from light.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or Fix/Lyse Solution with minimal brightness loss.
• Methanol Compatibility: Compatible with short-term methanol fixation (<1 hour).
• Excitation: 355 nm; Emission: 732 nm; Laser: Ultraviolet.

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells and constitutes ~10% of lymphocyte surface proteins. It regulates Src-family kinases and plays a role in lymphocyte development, antigen signaling, integrin-mediated survival, and apoptosis. CD45 isoforms differ by extracellular domains due to alternative splicing, with expression varying by cell type and state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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