Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 661
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells and at low levels on leukocytes and platelets. CD31 is involved in adhesion, cell-cell/matrix interaction, and signal transduction.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Tested on mouse bone marrow cells at ≤ 0.25 µg/test. One test = amount of antibody for staining a sample in 100 µL volume (10⁵–10⁸ cells/test). Optimize concentration per assay.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer-based dyes (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use

• Super Bright Complete Staining Buffer (SB-4401) or
• Brilliant Stain Buffer (00-4409)
  to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (00-8222)
• 1-step Fix/Lyse Solution (00-5333)

BUV737 is compatible with short-term methanol fixation (<1 hour).

Excitation/Emission:
355 nm / 732 nm (Ultraviolet Laser)

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T/B cell signaling via ITIM motifs. It is expressed in vascular compartments, primarily at cell junctions, mediating homophilic binding and cell-cell interactions. CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, and thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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