Thermo Fisher Scientific Nucleophosmin Monoclonal Antibody (NPM1, 1902)
NPM1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. Human 시료 반응성, Protein A/G 정제, 4°C 보관. 세포 내 NPM1 단백질 연구 및 종양 관련 단백질 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NPM1, 1902 |
| Immunogen | Recombinant fragment (around aa185–287) of human NPM1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
NPM1 (Nucleophosmin 1, also known as B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly and transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and p53 regulation.
It shuttles between the nucleus, cytoplasm, and nucleolus, chaperoning core histones and preventing protein aggregation.
NPM1 participates in several tumor-associated fusion proteins (NPM-ALK, NPM-RAR, NPM-MLF1) and is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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