Thermo Fisher Scientific Nucleophosmin Monoclonal Antibody (NPM1, 1902)
Thermo Fisher Scientific의 Nucleophosmin Monoclonal Antibody (NPM1, 1902)은 인간 NPM1 단백질을 타겟으로 하는 Mouse IgG2b 항체입니다. WB, IHC, ICC/IF 등 다양한 응용에 적합하며, 고순도 Protein A/G 정제 방식으로 제조되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 4869-MSM2-P1ABX
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NPM1, 1902 |
| Immunogen | Recombinant fragment (around aa185–287) of human NPM1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Tested in direct ELISA (Use antibody at 2–4 µg/mL for coating).
- For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly and transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication, and regulation of p53.
It continuously shuttles between the nucleus, cytoplasm, and nucleolus, chaperoning core histones and preventing protein aggregation. NPM1 also protects enzymes during thermal denaturation and assists in renaturation of denatured proteins.
NPM1 is associated with centrosome duplication and acts as a substrate of CDK2/cyclin E. It participates in several tumor-associated fusion proteins (NPM-ALK, NPM-RAR, NPM-MLF1) and is implicated in oncogenic processes.
Dysfunction of NPM1 is linked to diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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