Thermo Fisher Scientific CD47 Monoclonal Antibody (OTI6A2)

Applications

Flow Cytometry (Flow)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and regulates their functions. Engagement of CD47 modulates signaling pathways, including activation of heterotrimeric G proteins and intracellular calcium increase upon cell adhesion.
CD47 serves as a receptor for thrombospondin’s C-terminal domain and plays roles in membrane transport and signal transduction. It is broadly distributed across tissues and reduced on Rh erythrocytes.
CD47 acts as a ligand for CD172a (SIRPα), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. The CD47-CD172a interaction influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanical response, and neuronal development, especially in synapse-rich brain and retina regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.