Thermo Fisher Scientific CD47 Monoclonal Antibody (OTI6B2)

Applications

• Flow Cytometry (Flow): Tested dilution 1:100

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein that interacts with several integrins and regulates their functions. Engagement of CD47 by soluble ligands or counter receptors modulates signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in increasing intracellular calcium concentration upon cell adhesion to the extracellular matrix.
It also serves as a receptor for the C-terminal cell-binding domain of thrombospondin and may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, with reduced expression on Rh erythrocytes.
CD47 acts as a ligand for CD172a (SIRPα), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. The CD47–CD172a system also influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, particularly in synapse-rich brain and retinal regions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.