Thermo Fisher Scientific SHANK1 Monoclonal Antibody (N22/21), PerCP
SHANK1 단백질을 인식하는 PerCP 결합 단일클론 항체로, 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ICC, 항체 마이크로어레이에 사용 가능합니다. 단백질 G 정제, 보존제 없음, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N22/21 |
| Immunogen | Fusion protein amino acids 469–691 (SH3/PDZ domains) of rat Shank1 |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931902 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45448 was sufficient for detection of Shank1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 190–220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Target Information
SHANK1 is an adapter protein located in the postsynaptic density (PSD) of excitatory synapses. It interconnects postsynaptic membrane receptors such as NMDA-type and metabotropic glutamate receptors through complexes with GKAP/PSD-95 and Homer, linking them to the actin-based cytoskeleton. It plays a role in structural and functional organization of dendritic spines and synaptic junctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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