Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), PE-eFluor 610, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test
• View 160 publications

Functional Assay (Functional)

• Tested Dilution: –
• View 2 publications

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor series, FITC, PerCP, Super Bright series, NovaFluor series, PE, PE-Cyanine series, Functional Grade 등 다양한 형광체 및 비결합형 버전 제공.

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ heterodimer or CD8αα homodimer. CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a critical role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (defined as antibody amount per 100 µL cell sample). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Information:
PE-eFluor® 610 is excited by 488–561 nm laser lines and emits at 607 nm. Use a 610/20 band pass filter (PE-Texas Red® equivalent). Ensure instrument compatibility.

Handling Notes:

• Light sensitivity: Protect vial and stained samples from light.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).
• Filtration: 0.2 µm post-manufacturing filtered.

Excitation: 488–561 nm
Emission: 607 nm
Laser: Blue, Green, Yellow-Green Laser

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family, essential for T cell signal transduction, differentiation, and activation. CD8 predominantly exists on T cells as CD8αβ heterodimers functioning as co-receptors for MHC-I molecules. CD8α chain mediates MHC-I binding and is also found on NK cells, monocytes, and dendritic cells as CD8αα homodimers. MHC-I ligation recruits Lck kinase, leading to lymphokine production and cytotoxic T cell activation. Activated CTLs are critical for pathogen and tumor clearance. Differentiation of naïve CD8⁺ T cells is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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