Thermo Fisher Scientific OCT4 Monoclonal Antibody (5F7)
인간 OCT4 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 ELISA에 적합합니다. 줄기세포 연구 및 iPS 세포 생성 관련 연구에 활용됩니다. 비결합형 액상 형태이며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| ELISA | 1:10,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F7 |
| Immunogen | Synthesized peptide of human OCT4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10982966 |
Product Specific Information
MA5-15736 targets OCT4 in indirect ELISA and Western blot applications and shows reactivity with human samples.
It detects OCT4, which has a predicted molecular weight of approximately 45 kDa.
Target Information
POU5F1 (also known as Oct-4) is a maternally expressed octamer-binding protein and the first transcription factor described for early developmental stages.
Its role in embryonic development suggests its importance in stem cell generation for potential use in regenerative medicine.
Induced pluripotent stem (iPS) cells can be created by expressing POU5F1 along with transcription factors Sox2, Klf4, Lin28, and c-Myc in mouse fibroblasts.
Recent studies have shown that iPS cells can also be generated using expression plasmids without viral vectors, improving safety for regenerative applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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