Thermo Fisher Scientific Carbonic Anhydrase IX Monoclonal Antibody (2D3)
인간 CA9 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, Flow, ELISA 등 다양한 응용에 적합. Hypoxia 관련 연구 및 CA IX 발현 분석에 유용. 비결합형 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Thermo Fisher Scientific Carbonic Anhydrase IX Monoclonal Antibody (2D3)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D3 |
| Immunogen | Purified recombinant fragment of human CA9 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10984005 |
Product Specific Information
MA5-15737 targets CA9 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CA9 expressed in E. coli.
MA5-15737 detects CA9, which has a predicted molecular weight of approximately 50 kDa.
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in mammalian tissues and plays a key role in pH regulation through hydrogen ion (H⁺) flux.
CA IX is highly inducible under hypoxic conditions via HIF-1α and is a reliable histochemical marker of hypoxia. Blockade of CA IX increases cell death under hypoxia, highlighting its importance in hypoxia-related cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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