Thermo Fisher Scientific SOX2 Monoclonal Antibody (10F10)
인간 SOX2 단백질을 검출하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF, ELISA, ChIP 등 다양한 응용에 적합. 정제된 재조합 SOX2 단백질을 면역원으로 사용. 줄기세포 유지 및 발달 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
| ChIP assay (ChIP) | 4 µl / 10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10F10 |
| Immunogen | Purified recombinant fragment of human SOX2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10980318 |
Product Specific Information
MA5-15734 targets SOX2 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human SOX2 expressed in E. coli.
MA5-15734 detects SOX2, which has a predicted molecular weight of approximately 34 kDa.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in embryonic development and cell fate determination.
The SOX2 protein is required for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach.
The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
SOX2 protein may function as a transcriptional activator through complex formation with other proteins.
Mutations in SOX2 are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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