Thermo Fisher Scientific USP13 Monoclonal Antibody (OTI4G1), TrueMAB
USP13 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC(P), IP에 사용 가능. Human 시료 반응. Lyophilized 형태, PBS(pH 7.3) + 8% trehalose buffer. -20°C 보관, 동결/해동 반복 피해야 함.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunoprecipitation (IP) | 4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G1 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human USP13 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
USP13 is a deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2, and USP10. It participates in processes including autophagy and endoplasmic reticulum-associated degradation (ERAD).
It regulates autophagy and p53/TP53 levels by stabilizing BECN1 and USP10, forming a regulatory loop with PIK3C3/VPS34-containing complexes.
USP13 also mediates deubiquitination of SKP2 and UBL4A, influencing ERAD, and stabilizes SIAH2 by inhibiting its autoubiquitination.
It shows weak deubiquitinase activity in vitro, preferentially cleaving Lys-63-linked polyubiquitin chains. Unlike USP5, it cannot disassemble unanchored polyubiquitin chains. It can cleave ISG15 in vitro, though further validation is needed. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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