Thermo Fisher Scientific IL-17A Polyclonal Antibody, HRP

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.
• In ELISA and other immunoreactive assays, this antibody recognizes both native and recombinant human IL-17A in cell supernatants and certain body fluids.
• A control of similarly diluted normal rabbit IgG is recommended.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer consisting of 136 amino acids and belongs to the IL-17 family (IL-17A–F). It signals through the IL-17 receptor (IL-17R/CDw217).
High levels of IL-17A are produced by activated CD4+ T-cells and enhance ICAM-1 expression in human fibroblasts. IL-17A also stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2.
In the presence of IL-17A, fibroblasts can sustain proliferation of CD34+ hematopoietic progenitors and induce neutrophil maturation.
Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, showing cross-reactivity among homologs.
IL-17A regulates NF-κB and MAP kinase activities, stimulates IL-6 and COX-2 expression, and enhances nitric oxide (NO) production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.