Thermo Fisher Scientific NME4 Monoclonal Antibody (OTI4E8), TrueMAB
NME4 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI4E8). Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4E8 |
| Immunogen | Full length human recombinant protein of human NME4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
NME4 is a protein kinase that transfers phosphate groups from ATP to substrate proteins. This phosphorylation process mediates signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle progression, cytoskeletal organization, cell movement, apoptosis, and differentiation.
Protein kinases constitute one of the largest protein families in eukaryotes, with over 500 gene products, and are classified into eight major groups based on catalytic domain sequences. The AGC kinase group includes PKA, PKG, PKC, Akt, ARK, and RSK families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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