Thermo Fisher Scientific Phospho-DNAJC2 (Ser49) Polyclonal Antibody
DNAJC2 단백질의 Ser49 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 면역세포염색에 적합하며, 인산화된 단백질 검출에 유용. 1 mg/mL 농도의 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DNAJC2 (Accession Q99543), corresponding to amino acid residues around phosphorylated Ser49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817120 |
Product Specific Information
This antibody detects endogenous levels of DNAJC2/MPP11 only when phosphorylated at Ser49.
Target Information
EIF4EBP1 is a member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction with eIF4E inhibits complex assembly and represses translation. This protein becomes phosphorylated in response to various signals, including UV irradiation and insulin signaling, leading to dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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