Thermo Fisher Scientific Phospho-gamma Catenin (Tyr550) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-gamma-Catenin (Tyr-550) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 550 of human gamma-Catenin. This peptide sequence is highly conserved in rat and mouse gamma-Catenin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942896

Product Specific Information

This antibody was cross-adsorbed to both phospho-tyrosine coupled to agarose and to dephosphorylated gamma-Catenin (Tyr-550) peptide before affinity purification using phospho-gamma-Catenin (Tyr-550) peptide (without carrier). The antibody detects an 84kDa* protein corresponding to the molecular mass of gamma-Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.

Target Information

JUP is a major cytoplasmic protein which is the only known constituent common to sub membranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.