Thermo Fisher Scientific Phospho-Cofilin (Ser3) Polyclonal Antibody
Phospho-Cofilin (Ser3) 폴리클로날 항체로 인간, 생쥐, 랫트에 반응하며 Western blot과 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 항체로, 세포골격 조절 및 actin 동역학 연구에 적합. -20°C에서 보관.
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Thermo Fisher Scientific Phospho-Cofilin (Ser3) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phospho-peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 3 in human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942897 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated Cofilin 1 peptide then affinity purified using phospho-Cofilin 1 (Ser-3) peptide (without carrier). The antibody detects a 19 kDa protein corresponding to the molecular mass of phosphorylated Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells. This band can be removed by lambda phosphatase treatment.
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. It regulates actin cytoskeleton dynamics and is important for normal progress through mitosis and cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Also essential for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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