Thermo Fisher Scientific USP9X Monoclonal Antibody (OTI1D7), TrueMAB
USP9X 단백질을 인식하는 Mouse monoclonal antibody로 Western blot에 최적화됨. Human 유래 단백질과 반응하며, Lyophilized 형태로 제공. 재구성 후 1 mg/mL 농도로 사용 가능하며, 연구용으로 적합.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2246–2570 of human USP9X produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
USP9X is a ubiquitin-specific protease that removes and recycles ubiquitin from proteins degraded in proteasomes. It plays a critical role in tight junction biogenesis by regulating the levels of EFA6, a protein essential for tight junction formation during cell polarity establishment.
Located on the X chromosome, USP9X escapes X-inactivation, and defects in this gene may lead to gonadal degeneration in Turner syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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