Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI3B7), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that enable conjugation of Ub and Ub-like molecules to specific protein substrates.

1. Activation: ATP-dependent activation of the Ub C-terminus by the E1 enzyme.
2. Conjugation: Formation of an intermediate Ub–E2 complex via the Ub-conjugating enzyme (E2).
3. Ligation: Transfer of Ub from E2 to the target protein substrate catalyzed by the Ub-ligase (E3).

UBE2E3 (also known as UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and plays a role in regulating transepithelial sodium transport in renal cells. It may also be involved in cell growth arrest. UBE2E3 shuttles between the cytoplasm and nucleus in an IPO11-dependent manner and is ubiquitously expressed at low levels, with high expression in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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