Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

Description:
The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.

Applications Reported:
This antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood.
Use isotype control at the same concentration as the experimental antibody.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The isotype of a primary antibody and the application it is used in can result in background staining.
Primary antibody background noise can be caused by binding to Fc receptors, non-specific interactions, or cell autofluorescence.
Isotype control antibodies act as negative controls to differentiate non-specific background signal from specific antibody signal.
They are most commonly used in flow cytometry but also useful in ChIP, immunohistochemistry, and gel shift assays.
Isotype controls should match the primary antibody species and isotype, and when using directly labeled antibodies, the control should have the same conjugate label.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.