Thermo Fisher Scientific Syrian Hamster IgG Isotype Control, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum.

Applications Reported:
Golden Syrian hamster IgG isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemical staining, immunoprecipitation, and immunoblotting (WB).

Applications Tested:
This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use the isotype control at the same concentration as the experimental antibody.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells, non-specific interactions with cellular proteins, carbohydrates, and lipids, or by cell autofluorescence.

Isotype control antibodies serve as negative controls to differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.

While isotype controls are most commonly used in flow cytometry, they are also useful in applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match the primary antibody species and isotype so that specific staining can be accurately determined.

If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.