Thermo Fisher Scientific PCNA Monoclonal Antibody (7H4F8)
PCNA 단백질을 인식하는 mouse monoclonal antibody로, WB, IHC, Flow, ELISA에 적합합니다. Human, Mouse, Rat 시료에 반응하며, Protein G 정제 및 PBS buffer에 0.05% sodium azide가 포함되어 있습니다. 연구용으로 DNA 복제 및 세포 증식 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7H4F8 |
| Immunogen | Purified recombinant fragment of human PCNA (amino acids 53–196) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538616 |
Product Specific Information
MA5-17145 targets PCNA in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human PCNA (amino acids 53–196) expressed in E. coli.
MA5-17145 detects PCNA with a predicted molecular weight of approximately 28.7 kDa.
Target Information
PCNA (proliferating cell nuclear antigen) is essential for DNA replication and participates in DNA excision and mismatch repair pathways.
It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker of proliferating cells and helps assess tumor proliferation status.
It is localized in the nucleus, functions as a cofactor of DNA polymerase delta, and forms a homotrimer to enhance leading strand synthesis during replication.
Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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