Thermo Fisher Scientific NPM1 Monoclonal Antibody (7H10B9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7H10B9

Immunogen

Purified recombinant fragment of human NPM (2-265) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NPM1,uniProtId:P06748-1,ncbiNodeId:9606,antigenRange:2-265,antigenLength:294,antigenImageFileName:MA5-17141_NPM1_P06748-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17141_NPM1_P06748-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538612

Product Specific Information

MA5-17141 targets Nucleophosmin in indirect ELISA, ICC, IHC, IF and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-17141 immunogen is purified recombinant fragment of human NPM (2-265) expressed in E. Coli.

MA5-17141 detects Nucleophosmin which has a predicted molecular weight of approximately 33kDa.

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.