Thermo Fisher Scientific iNOS Monoclonal Antibody (4E5)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MA5-17139 targets NOS2 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples.
The MA5-17139 immunogen is purified recombinant fragment of human NOS2 expressed in E. coli.
MA5-17139 detects NOS2, which has a predicted molecular weight of approximately 131 kDa.

Target Information

iNOS (Inducible Nitric Oxide Synthase, NOS) is an inorganic, gaseous free radical that mediates various intercellular signals including vasorelaxation, neurotransmission, and cytotoxicity.
NO production is catalyzed by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by lipopolysaccharide and cytokines.
NOS catalyzes the oxidation of L-arginine to produce L-citrulline and NO. Three isoforms have been cloned: neuronal NOS (nNOS, type I), endothelial NOS (eNOS, type III), and inducible NOS (iNOS, type II).
iNOS contains calmodulin, NADPH, FAD, and FMN binding domains and is found in macrophages, hepatocytes, synoviocytes, and smooth muscle cells.
Cytokines such as IFN-γ, TNF, IL-1, IL-2, and LPS increase iNOS mRNA, protein, and activity levels, leading to sustained NO production.
Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.