Thermo Fisher Scientific KIT Monoclonal Antibody (OTI2B5), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for mast cell growth factor (MGF, also known as stem cell factor). It was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit.
KIT and its ligand regulate growth and activation of various hematopoietic and non-hematopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.

Deregulation of KIT receptor tyrosine kinase by mutation D816V is a key target in therapeutic strategies against neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells.

Upon binding of its ligand SCF, c-Kit dimerizes and autophosphorylates tyrosine residues such as Tyr703, enabling Grb2 docking and activation of the Ras/ERK pathway. SCF/c-Kit signaling also activates PI3K, PLC-γ, and JAK/STAT pathways, which are essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.