Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 649
GFP 전체 서열을 면역원으로 한 염소 폴리클로날 항체로 DyLight 649 형광 표지. Western blot, ICC/IF, ELISA에 적합하며 높은 특이성과 감도를 제공. 651/673 nm 형광 특성으로 다양한 형광 이미징 응용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | 1:20,000–1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full-length amino acid sequence (246aa) derived from Aequorea victoria |
| Conjugate | DyLight™ 649 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing.
- Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified/partially purified GFP (Aequorea victoria).
No cross-reactivity observed with Human, Mouse, or Rat serum proteins.
Target Information
The jellyfish Aequorea victoria produces Green Fluorescent Protein (GFP), which emits green light during bioluminescence. GFP is widely used as a reporter protein for gene expression and as a protein tag in both prokaryotic and eukaryotic systems.
As a fusion tag, GFP enables visualization of protein localization, dynamics, and subcellular compartment behavior. GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically, absorbing maximally at 395 nm and emitting at 508 nm. Its fluorescence is stable, species-independent, and does not require cofactors, making it ideal for diverse applications such as:
- Monitoring gene expression
- Protein localization studies
- Protein–protein interaction assays (e.g., yeast two-hybrid)
- FRET-based distance measurements
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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