Thermo Fisher Scientific GFP Polyclonal Antibody, DyLight 680

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot and freeze at -20°C or below.
• Avoid freeze/thaw cycles.
• Centrifuge product if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
No reaction was observed against Human, Mouse, or Rat serum proteins.

Target Information

The jellyfish Aequorea victoria contains Green Fluorescent Protein (GFP Tag) that emits light in the bioluminescence reaction.
GFP is widely used as a reporter protein in gene expression studies and as a protein tag in both eukaryotic and prokaryotic systems.
As a fusion tag, GFP enables localization and movement studies of proteins and investigation of subcellular dynamics.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autocatalytically, absorbing blue light (395 nm) and emitting green light (508 nm).
It is stable, species-independent, and suitable for diverse applications such as monitoring gene expression, protein localization, protein–protein interactions (yeast two-hybrid), and FRET experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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